KMID : 1033620180450040177
|
|
Clinical and Experimental Reproductive Medicine 2018 Volume.45 No. 4 p.177 ~ p.182
|
|
Increased cryo-survival rate in ejaculated human sperm from infertile men following pre-freeze in vitro myo-inositol supplementation
|
|
Saleh Ramadan
Assaf Hanan El Maged Wafaa M. Abd Elsuity Mohamed Fawzy Mohamed
|
|
Abstract
|
|
|
Objective: To investigate the effects of in vitro myo-inositol (Myo-Ins) supplementation of cryopreserved human semen on the cryo-survival rate (CSR).
Methods: Semen samples were obtained from 41 infertile men. Following routine semen analysis, each sample was divided into two equal aliquots (0.5 mL each). One aliquot was treated with 1 mg of Myo-Ins dissolved in 10 ¥ìL of sperm preparation medium. The second aliquot was treated with 10 ¥ìL of the same medium (control). Both aliquots were incubated for 20 minutes prior to freezing to slow the freezing process. The frozen samples were examined for post-thaw percentages of total motility (TM), progressive motility (PM), and the CSR, defined as the percentage of post-thaw TM divided by the percentage of pre-freeze TM and multiplied in 100. The results were expressed as median and interquartile range (25th and 75th percentiles).
Results: The pre-freeze TM (50% [30%?50%]) and PM (35% [20%?35%]) were significantly higher than the post-thaw TM and PM in the Myo-Ins group (15% [10%?35%] and 10% [5%?20%]; p<0.001 and p<0.001, respectively) and the control group (10% [6%?30%] and 5% [3%?15%]; p<0.001 and p<0.001, respectively). The CSR of the 41 semen aliquots supplemented with Myo-Ins (40% [25%?70%]) was significantly higher than that of the control samples (30% [13%?58%], p=0.041). The CSR of the 26 abnormal semen samples that were supplemented with Myo-Ins (38% [20%?50%]) was significantly higher than that of the control samples (23% [12%?30%], p=0.031).
Conclusion: In vitro Myo-Ins supplementation of ejaculated human sperm from infertile men resulted in a significant increase in the CSR in samples with abnormal pre-freeze sperm parameters.
|
|
KEYWORD
|
|
Cryo-survival rate, Human sperm, Infertile men, Myo-inositol, Sperm cryopreservation
|
|
FullTexts / Linksout information
|
|
|
|
Listed journal information
|
|
|